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Article Dans Une Revue Radiation Research Année : 2023

RENEB Inter-Laboratory Comparison 2021: The Dicentric Chromosome Assay

1 BfS - Bundesamt für Strahlenschutz - Federal Office for Radiation Protection
2 Bundeswehr Institute of Radiobiology
3 UKHSA - UK Health Security Agency [London]
4 IRSN/PSE-SANTE/SERAMED/LRAcc - Laboratoire de Radiobiologie des expositions accidentelles
5 UGENT - Universiteit Gent = Ghent University
6 Armed Forces Biomedical Research Institute of Medical Science
7 Hospital Universitari i Politècnic La Fe = University and Polytechnic Hospital La Fe
8 French National Centre for Scientific Research
9 Centro de Ciencias e Tecnologias Nucleares, Instituto Superior Tecnico, Universidade de Lisboa, Bobadela LRS, Portugal
10 Hospital General Universitario "Gregorio Marañón" [Madrid]
11 LNL - Laboratori Nazionali di Legnaro
12 UAB - Universitat Autònoma de Barcelona = Autonomous University of Barcelona = Universidad Autónoma de Barcelona
13 National center for public health [Hungary]
14 ENEA - Agenzia Nazionale per le nuove Tecnologie, l’energia e lo sviluppo economico sostenibile = Italian National Agency for New Technologies, Energy and Sustainable Economic Development
15 INCT - Institute of Nuclear Chemistry and Technology
16 NCRRP - National Centre of Radiobiology and Radiation Protection, Sofia
17 Radiation Protection Centre
18 Universidad de Sevilla = University of Seville
19 Nükleer Arş Ens
20 Serbian Institute of Occupational Health, Radiation Protection Center
21 Commissariat à l'Energie Atomique (CEA), DRF/PROCyTOX , Fontenay-aux-Roses , France.
22 Department of Physiology and Radiobiology
23 GenEvolutioN
24 Consumer and Clinical Radiation Protection Bureau
25 Korea Institute of Radiological & Medical Sciences
26 ORISE - Oak Ridge Institute for Science and Education
27 Radiation Cytogenetics Laboratory
28 Dalat Nuclear Research Institute
29 Hirosaki University
30 QST - National Institutes for Quantum and Radiological Science and Technology
31 Fukushima Medical University School of Medicine
32 Atomic Bomb Disease Institute
33 Singapore Nuclear Research and Safety Initiativ
I. Güçlü
  • Fonction : Auteur
F. Finot
  • Fonction : Auteur
C. Cuceu Petrenci
  • Fonction : Auteur
T. Miura
  • Fonction : Auteur

Résumé

After large-scale radiation accidents where many individuals are suspected to be exposed to ionizing radiation, biological and physical retrospective dosimetry assays are important tools to aid clinical decision making by categorizing individuals into unexposed/minimally, moderately or highly exposed groups. Quality-controlled inter-laboratory comparisons of simulated accident scenarios are regularly performed in the frame of the European legal association RENEB (Running the European Network of Biological and Physical retrospective Dosimetry) to optimize international networking and emergency readiness in case of large-scale radiation events. In total 33 laboratories from 22 countries around the world participated in the current RENEB inter-laboratory comparison 2021 for the dicentric chromosome assay. Blood was irradiated in vitro with X rays (240 kVp, 13 mA, ∼75 keV, 1 Gy/min) to simulate an acute, homogeneous whole-body exposure. Three blood samples (no. 1: 0 Gy, no. 2: 1.2 Gy, no. 3: 3.5 Gy) were sent to each participant and the task was to culture samples, to prepare slides and to assess radiation doses based on the observed dicentric yields from 50 manually or 150 semi-automatically scored metaphases (triage mode scoring). Approximately two-thirds of the participants applied calibration curves from irradiations with γ rays and about 1/3 from irradiations with X rays with varying energies. The categorization of the samples in clinically relevant groups corresponding to individuals that were unexposed/minimally (0–1 Gy), moderately (1–2 Gy) or highly exposed (>2 Gy) was successfully performed by all participants for sample no. 1 and no. 3 and by ≥74% for sample no. 2. However, while most participants estimated a dose of exactly 0 Gy for the sham-irradiated sample, the precise dose estimates of the samples irradiated with doses >0 Gy were systematically higher than the corresponding reference doses and showed a median deviation of 0.5 Gy (sample no. 2) and 0.95 Gy (sample no. 3) for manual scoring. By converting doses estimated based on γ-ray calibration curves to X-ray doses of a comparable mean photon energy as used in this exercise, the median deviation decreased to 0.27 Gy (sample no. 2) and 0.6 Gy (sample no. 3). The main aim of biological dosimetry in the case of a large-scale event is the categorization of individuals into clinically relevant groups, to aid clinical decision making. This task was successfully performed by all participants for the 0 Gy and 3.5 Gy samples and by 74% (manual scoring) and 80% (semiautomatic scoring) for the 1.2 Gy sample. Due to the accuracy of the dicentric chromosome assay and the high number of participating laboratories, a systematic shift of the dose estimates could be revealed. Differences in radiation quality (X ray vs. γ ray) between the test samples and the applied dose effect curves can partly explain the systematic shift. There might be several additional reasons for the observed bias (e.g., donor effects, transport, experimental conditions or the irradiation setup) and the analysis of these reasons provides great opportunities for future research. The participation of laboratories from countries around the world gave the opportunity to compare the results on an international level.

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irsn-04243665 , version 1 (16-10-2023)

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D. Endesfelder, U. Oestreicher, M. Bucher, C. Beinke, C Siebenwirth, et al.. RENEB Inter-Laboratory Comparison 2021: The Dicentric Chromosome Assay. Radiation Research, 2023, 199 (6), pp.556-570. ⟨10.1667/RADE-22-00202.1⟩. ⟨irsn-04243665⟩
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