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Article Dans Une Revue Bioelectricity Année : 2023

Deciphering Immediate Post-Pulse Membrane Resealing from 4-Electrode Impedance Measurements by Numerical Modeling

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Background and Objective: The present paper proposes a numerical strategy to decipher the dynamics of the cell membranes exposed to an electroporating electric field from bioimpedance measurements. In particular we aim at discriminating between the increase of membrane conductivity due to electroporation from the increase of buffer conductivity due to ion exchange between buffer and cells. Methods: We propose first a robust calibration procedure that enables to account for the complexity of the 4--electrode experimental set-up. Thanks to this robust calibration, we deduce the impedance of the sample from the measurements. Then we propose a simple electrical circuit model of the set-up, which is calibrated into two steps. Results: First we estimate the model parameters before the electroporating electric field, in order to obtain the cell parameters. The dynamic of the membrane resistance after the pulses is then calibrated simultaneously with the increase of the buffer conductivity due to ions exchanges. Interestingly, our model and our calibration strategy enable us to capture the dynamics of the cell membranes within a few seconds after the pulse. For longer times, we explain how additional measurements of the buffer conductivity should be performed to track the dynamics of the membrane resealing more accurately. Conclusions: The combination of the robust calibration with the well-designed equivalent circuit model enables us to capture the dynamics of ions exchange and membrane permeabilisation within the few seconds after the electric pulse.
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hal-04361087 , version 1 (22-12-2023)

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Annabelle Collin, Tomás García-Sánchez, Sergio Corridore, Lluis Mir, Clair Poignard. Deciphering Immediate Post-Pulse Membrane Resealing from 4-Electrode Impedance Measurements by Numerical Modeling. Bioelectricity, 2023, 5 (4), pp.266-278. ⟨10.1089/bioe.2023.0012⟩. ⟨hal-04361087⟩
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