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Article Dans Une Revue Optics Letters Année : 2023

Sparse denoising and adaptive estimation enhances the resolution and contrast of fluorescence emission difference microscopy based on array detector

Résumé

Array detector allows a resolution gain for confocal microscopy by combining images sensed by a set of photomultipliers tubes (or sub-detectors). Several methods have been proposed to reconstruct a high-resolution image by linearly combining sub-detector images, especially the fluorescence emission difference (FED) technique. To improve the resolution and contrast of FED microscopy based on array detector, we propose to asso-ciate sparse denoising with spatial adaptive estimation. We show on both calibration slides and real data that our approach applied to the full stack of spatially reassigned detector signals, enables to achieve a higher reconstruction performance in terms of resolution, image contrast, and noise reduction.
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Dates et versions

hal-03931575 , version 1 (09-01-2023)

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Sylvain Prigent, Stéphanie Dutertre, Aurélien Bidaud-Meynard, Giulia Bertolin, Grégoire Michaux, et al.. Sparse denoising and adaptive estimation enhances the resolution and contrast of fluorescence emission difference microscopy based on array detector. Optics Letters, 2023, 48 (2), pp.1-11. ⟨10.1364/OL.474883⟩. ⟨hal-03931575⟩
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