At present, more and more companies are aware of and have the incentive to promote the construction and development of collaborative platforms, to improve collaboration to cope with many uncertainties such as market turmoil and increased competition. At the same time, with the emergence of emerging digital technologies, the concept of a digital business ecosystem is becoming more and more prominent. DBE development is a long-term evolutionary process, and there are many barriers in the practice. The current research on DBE is mainly conceptual and theoretical, and the barriers in the development process of DBE in the practice are still lack of exploration. Therefore, the purpose of this study is to summarize and analyze the key barriers in the practice of the enterprise through empirical investigation. According to the case companies’ mitigation measures and related literature research, we proposed some relevant suggestions and research ideas to solve the barriers.