Knowledge Intensive Organizations (KIOs) are of growing importance worldwide. But comparing to other kinds of enterprises, they are challenged by more uncertainty and complex situations. In the context of a KIO, various small teams can be dynamically organized to collaboratively pursue knowledge creation initiatives. As these small teams, which bred from KIOs, often involve activities that cannot be fully predetermined, they need to be supported by related resources and services in a more agile way during their operation and evolution stages. This paper presents a new three-layer collaboration framework for agile operation of small teams on the basis of existing models from the paradigm of Collaborative Networks, with a set of roles and mechanisms proposed in each layer. Considering the framework, related digital platforms and artificial intelligence applications can be developed, giving effective support to small teams’ knowledge creation processes. A case study has been developed for a research group to illustrate the proposed framework.