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Conference Papers Year : 2019

Isoprenoid metabolism and its impact on seed quality

Abstract

Seed nutritional and physiological quality, i.e. seed longevity, dormancy, germination and vigor are of predominant interest. These traits are tightly regulated during seed development and mainly rely on the accumulation of metabolites, including isoprenoids such as hormones (brassinosteroids, gibberellins, abscisic acid), sterols, carotenoids and tocopherols1,2. Isoprenoids derive from the C5 prenyl diphosphates isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), that are synthesized either by the cytosolic mevalonate (MVA) or the plastidial methylerythritol 4-phosphate (MEP) pathways. Exchanges of IPP and DMAPP between cell compartments occur at a very limited rate but would be more intense during seed development3, while being poorly understood. Isoprenoid metabolism is partly under the control of retrograde signaling, i.e. signals travelling from the plastid or the cytosol to the nucleus to regulate gene expression4, 5. Recently, a relationship between seed quality and coadaptation of nuclear and cytoplasmic (i.e. plastidial and mitochondrial) genomes has been discovered using Arabidopsis thaliana cytolines, an innovative plant material that combines nuclear and cytoplasmic genomes from different accessions and, hence, disrupts co-adaptation between natural variants of these genomes6. To decipher how plastid-nuclear interactions impact isoprenoid metabolism in relation to seed quality, we are screening the cytolines for altered IPP/DMAPP exchange fluxes during seed and seedling development using inhibitors of the MEP and MVA pathways. A first screening revealed that cytonuclear combination does affect the rate of IPP/DMAPP exchange after germination, during early seedling development. On a second part, we are characterizing the nutritional and physiological qualities of the seeds of the cytolines but also of transgenic lines overexpressing genes coding for key enzymes of MEP and MVA pathways as well as down-stream isoprenoids pathway like PHYTOENE SYNTHASE (PSY). Interestingly, overexpressing MEP pathway genes led to an increase in seed tocopherols but not in carotenoids. Identifying how isoprenoid metabolism is regulated during seed development and what is its impact on seed quality will be of interest to produce seeds of increased nutritional and physiological qualities, thus dealing with food security and nutrition challenges.
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Dates and versions

hal-03226289 , version 1 (14-05-2021)

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Attribution - NonCommercial - NoDerivatives

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  • HAL Id : hal-03226289 , version 1

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Julia Zinsmeister, Henk W. M. Hilhorst, Loïc Rajjou, Francoise Budar, Manuel Rodriguez-Concepcion. Isoprenoid metabolism and its impact on seed quality. XII meeting of SFBV (French Society of Plant Biology), French Society of Plant Biology (SFBV), Jul 2019, Strasbourg, France. ⟨hal-03226289⟩
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