The strategy of “one belt one road” has provided an opportunity of accelerate economic growth for China’s surrounding countries. Laos is actively joining the initiative, but how to cooperate with China is a difficulty. Biological symbiosis shares similar characters with cooperation and mutual benefits among cross-border countries. So taking the construction of Savan-Seno Special Economic Zone of Laos as example, we introduced the symbiosis theory and analyzing symbiosis units, models and interfaces between Laos-China regional economic cooperation, it led to the generation, optimization and mechanism under which the symbiotic system was determined. Then we should improve the Laos-China regional economic cooperation symbiosis system by training and harvesting favorable environments, expanding symbiotic interface so as to accelerate the cooperative relationship.