According to the low yield of edible fungus and the poor quality of edible fungi in factory, the production management situation in edible fungi factory was analyzed. Then the solution of edible fungi factory HACCP(Hazard Analysis Critical Control Point) MIS(management information system) based on RFID(Radio Frequency Identification) technology was presented. Edible fungi production process by the hazard analysis to determine the raw materials, burden mix materials and bottling, inoculation, culture, and growth as the critical control points. For each critical control points, respectively, to develop appropriate critical limits, clearly defined monitoring objects, methods, frequency and corrective measures, critical control point of information collection for the use of RFID technology. Through the implementation of HACCP MIS, edible fungi yield and quality have improved.