According to the disadvantages of traditional box for preservation, including low oxygen and high carbon, a new control system of high oxygen is designed. This paper presents the design and implementation method in this system. The system combines traditional PID control with fuzzy control which can adjust parameters of the whole system automatically. The box can control the dynamic content of oxygen and carbon dioxide, and monitor nitrogen flow, temperature and humidity real-timely so that preservation time can be prolonged. It also can collect and keep the data of the dynamic content of oxygen and carbon dioxide which suits for fruit and vegetable for preservation. The former environment can be reappeared when need. The system works steadily and has strong functions.