DGraph: Algorithms for Shortgun Reads Assembly Using De Bruijn Graph - Inria - Institut national de recherche en sciences et technologies du numérique Access content directly
Conference Papers Year : 2012

DGraph: Algorithms for Shortgun Reads Assembly Using De Bruijn Graph


Massively parallel DNA sequencing platforms have become widely available, reducing the cost of DNA sequencing by over two orders of magnitude, and democratizing the field by putting the sequencing capacity of a major genome center in the hands of individual investigators. New challenges include the development of robust protocols for generating sequencing libraries, building effective new approaches to resequence and data-analysis. In this paper we demonstrate a new sequencing algorithm, named DGraph, which has two modules, one module is responsible to construct De Bruijn graph by cutting reads into k-mers, and the other’s duty is to simplify this graph and collect all long contigs. The authors didn’t adapt the sequence graph reductions operations proposed by RAMANA M.IDURY or Finding Eulerian Superpaths proved by Pavel A.Pevzner or bubble remove steps suggested by Danial Zerbino, As the first operations was computing expensive, and the second one was impractical, and the last one did not benefit either the quality of contigs or the efficiency of the assembler. Our assembler was focused only on efficient and effective error removal and path reduction operations. Applying DGraph to the simulation data of fruit fly Drosophila melanogaster chromosome X, DGraph (3min) is about six times faster than velvet 0.3 (19 mins), and its coverage (92.5%) is also better than velvet (78.2%) when k = 21. Compare to velvet, the results shows that the algorithm of DGraph is a faster program with high quality results.
Fichier principal
Vignette du fichier
978-3-642-35606-3_2_Chapter.pdf (484.04 Ko) Télécharger le fichier
Origin : Files produced by the author(s)

Dates and versions

hal-01551353 , version 1 (30-06-2017)





Jintao Meng, Jianrui Yuan, Jiefeng Cheng, Yanjie Wei, Shengzhong Feng. DGraph: Algorithms for Shortgun Reads Assembly Using De Bruijn Graph. 9th International Conference on Network and Parallel Computing (NPC), Sep 2012, Gwangju, South Korea. pp.14-21, ⟨10.1007/978-3-642-35606-3_2⟩. ⟨hal-01551353⟩
53 View
161 Download



Gmail Facebook X LinkedIn More