Pan-retinal characterisation of Light Responses from Ganglion Cells in the Developing Mouse Retina
Abstract
We have investigated the ontogeny of light-driven responses in mouse retinal ganglion cells (RGCs).
Using a large-scale, high-density multielectrode array, we recorded from hundreds to thousands of
RGCs simultaneously at pan-retinal level, including dorsal and ventral locations. Responses to different
contrasts not only revealed a complex developmental profile for ON, OFF and ON-OFF RGC types,
but also unveiled differences between dorsal and ventral RGCs. At eye-opening, dorsal RGCs of all
types were more responsive to light, perhaps indicating an environmental priority to nest viewing for
pre-weaning pups. The developmental profile of ON and OFF RGCs exhibited antagonistic behavior,
with the strongest ON responses shortly after eye-opening, followed by an increase in the strength of
OFF responses later on. Further, we found that with maturation receptive field (RF) center sizes
decrease, responses to light get stronger, and centers become more circular while seeing differences in
all of them between RGC types. These findings show that retinal functionality is not spatially
homogeneous, likely reflecting ecological requirements that favour the early development of dorsal
retina, and reflecting different roles in vision in the mature animal.
Origin : Files produced by the author(s)
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