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Phase Estimation in Differential-Interference-Contrast (DIC) Microscopy


We present a gradient-based optimization method for the estimation of a specimen's phase function from polychromatic DIC images. The method minimizes the sum of a nonlinear least-squares discrepancy measure and a smooth approximation of the total variation. A new formulation of the gradient and a recent updating rule for the choice of the step size are both exploited to reduce computational time. Numerical simulations on two computer-generated objects show significant improvements, both in efficiency and accuracy, with respect to a more standard choice of the step size.
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hal-01255004 , version 1 (14-01-2016)


  • HAL Id : hal-01255004 , version 1


Lola Bautista, Simone Rebegoldi, Laure Blanc-Féraud, Marco Prato, Luca Zanni, et al.. Phase Estimation in Differential-Interference-Contrast (DIC) Microscopy. IEEE International Symposium on Biomedical Imaging (ISBI), Apr 2016, Prague, Czech Republic. pp.136-139. ⟨hal-01255004⟩
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