Fermentation Condition Optimization for Endophytic Fungus BS002 Isolated from Sophora Flavescens by Response Surface Methodology
Abstract
The endophytic fungus BS002 with good effect of disease-resistant has
been isolated from the medicinal Sophora flavescens. The conditions of
the fermentation medium were explored in this paper. On the basis of the
single factor experiments, CH3COONa, potato and
glucose were defined to be the main factors by Plackett-Burman Design;
Response surface methodology (RSM) with Box-Behnke Design was used to
optimize fermentation conditions of endophytic fungi BS002. The optimal
conditions are defined as follows: 100 mL in the container 250mL, potato
246.47 g/L, glucose 27.81 g/L, CH3COONa 1.95 g/L,
concentration of fungus 107 cfu/ml,
temperature 25 oC, yeast extract 1.0 g/L,
speed 150 rpm, fermentation for 4 d, dry weight of mycelium can reach
10.398 g/L.
| Origin | Files produced by the author(s) |
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