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Conference Papers Year : 2014

A 3D model with shape prior information for biological structures reconstruction using Multiple-Angle Total Internal Reflection Fluorescence Microscopy

Emmanuel Soubies
Laure Blanc-Féraud
Sébastien Schaub
Gilles Aubert
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Abstract

We propose a new model for the reconstruction of biological struc- tures using Multiple-Angle Total Internal Reflection Fluorescence Microscopy (MA-TIRFM). This recent microscopy technique allows the visualization of sub-cellular structures around the plasma mem- brane which is of fundamental importance in the comprehension of exchanges mechanisms of the cell. We present a 3D reconstruction method based on a shape prior information on the observed struc- tures and robust to shot noise and background fluorescence. A nov- elty with respect to the state of the art is to propose a method allow- ing the recovery of multiple objects aligned along the axial axis. The optimization problem can be formulated as a minimization problem where both the number of objects in the model and their parame- ters have to be estimated. This difficult combinatorial optimization problem is tackled by using a Marked Point Process approach which allows modelling interactions between the objects in order to regu- larize the inverse problem. Finally, performances of the proposed method are evaluated on synthetic data and real data.
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Dates and versions

hal-00914998 , version 1 (06-12-2013)

Identifiers

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Emmanuel Soubies, Laure Blanc-Féraud, Sébastien Schaub, Gilles Aubert. A 3D model with shape prior information for biological structures reconstruction using Multiple-Angle Total Internal Reflection Fluorescence Microscopy. ISBI - International Symposium on Biomedical Imaging, Apr 2014, Beijing, China. pp.608 - 611, ⟨10.1109/ISBI.2014.6867944⟩. ⟨hal-00914998⟩
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