Modeling the impact of granular embedding media, and pulling versus pushing cells on growing cell clones - Inria - Institut national de recherche en sciences et technologies du numérique Access content directly
Journal Articles New Journal of Physics Year : 2012

Modeling the impact of granular embedding media, and pulling versus pushing cells on growing cell clones

Abstract

In this paper, we explore how potential biomechanical influences on cell cycle entrance and cell migration affect the growth dynamics of cell populations. We consider cell populations growing in free, granular and tissuelike environments using a mathematical single-cell-based model. In a free environment we study the effect of pushing movements triggered by proliferation versus active pulling movements of cells stretching cell-cell contacts on the multi-cellular kinetics and the cell population morphotype. By growing cell clones embedded in agarose gel or cells of another type, one can mimic aspects of embedding tissues. We perform simulation studies of cell clones expanding in an environment of granular objects and of chemically inert cells. In certain parameter ranges, we find the formation of invasive fingers reminiscent of viscous fingering. Since the simulation studies are highly computation-time consuming, we mainly study one-cell-thick monolayers and show that for selected parameter settings the results also hold for multi-cellular spheroids. Finally, we compare our model to the experimentally observed growth dynamics of multi-cellular spheroids in agarose gel.
Fichier principal
Vignette du fichier
DrasdoHoehmeNJP2012.pdf (1.29 Mo) Télécharger le fichier
Origin : Publisher files allowed on an open archive
Loading...

Dates and versions

hal-00778129 , version 1 (18-01-2013)

Identifiers

Cite

Dirk Drasdo, Stefan Hoehme. Modeling the impact of granular embedding media, and pulling versus pushing cells on growing cell clones. New Journal of Physics, 2012, 14, 055025 (37pp). ⟨10.1088/1367-2630/14/5/055025⟩. ⟨hal-00778129⟩
175 View
190 Download

Altmetric

Share

Gmail Facebook X LinkedIn More