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Conference Papers Year : 2010

Genome-scale Metabolic Reconstruction of the Eukaryote Cell Factory Yarrowia Lipolytica

Abstract

Yarrowia lipolytica is an oleaginous yeast studied experimentally for its role as a food contaminant and its use in bioremediation and cell factory applications. As one of the hemiascomycetous yeasts completely sequenced in the Génolevures program it enjoys a high quality manual annotation by a network of experts. We have developed a method of semi-automatic reconstruction of metabolic models, based on the prediction of conservation of enzymatic activities between two species. Following our reconstruction protocol, we extrapolated a Y.lipolytica genome-scale metabolic model (called iNL705) from an existing S.cerevisiae model (iND750). This draft model was curated by a group of experts in Y. lipolytica metabolism, and iteratively improved and validated through comparison with experimental data by flux balance analysis.
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Dates and versions

hal-00652922 , version 1 (16-12-2011)

Identifiers

  • HAL Id : hal-00652922 , version 1

Cite

Nicolás Loira, David James Sherman. Genome-scale Metabolic Reconstruction of the Eukaryote Cell Factory Yarrowia Lipolytica. 11th International Conference on Systems Biology, ICSB, Oct 2010, Edinburgh, United Kingdom. ⟨hal-00652922⟩
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