Detailed transcript map of a 810-kb region at 11p14 involving identification of 10 novel human 3' exons - Inria - Institut national de recherche en sciences et technologies du numérique Access content directly
Journal Articles European Journal of Human Genetics Year : 1999

Detailed transcript map of a 810-kb region at 11p14 involving identification of 10 novel human 3' exons

Fabrice Guillemot
  • Function : Author
Charles Auffray
  • Function : Author

Abstract

A limited number of genes, including the human brain-derived neutrotrophic factor (BDNF) gene, have been identified in the human chromosome 11p14 region. Since this area is involved in a genetic disorder (WAGR syndrome) and because of interest in studying the regulation of the human BDNF gene, we have established a detailed transcript map of a 810-kb region clone in a yeast artificial chromosome (YAC), corresponding to a portion of this genomic locus. A set of nested deletion mutants has been generated to map genes at a mean resolution of 75kb. Four genic markers from available mapping databases have been mapped on the YAC.Ten potential novel human exons have been isolated by a 3' terminal exon trapping procedure directly applied to purified YAC DNA. Most of these exons display polyadenylation signals and they all yield positive signals in RT-PCR experiments, confirming their status of transcribed sequences. The BDNF gene is now co-localised with three other genes on a 120 kb DNA fragment.
Not file

Dates and versions

inria-00098891 , version 1 (26-09-2006)

Identifiers

  • HAL Id : inria-00098891 , version 1

Cite

Fabrice Guillemot, Charles Auffray, Marie-Dominique Devignes. Detailed transcript map of a 810-kb region at 11p14 involving identification of 10 novel human 3' exons. European Journal of Human Genetics, 1999, 7 (4), pp.487-495. ⟨inria-00098891⟩
59 View
0 Download

Share

Gmail Facebook Twitter LinkedIn More