Water fluxes contribute to growth patterning in shoot meristems - Inria - Institut national de recherche en sciences et technologies du numérique Access content directly
Preprints, Working Papers, ... Year : 2023

Water fluxes contribute to growth patterning in shoot meristems

Abstract

Abstract In multicellular organisms, localized tissue outgrowth creates a new water sink thereby modifying hydraulic patterns at the organ level. These fluxes are often considered passive by-products of development and their patterning and potential contribution to morphogenesis remains largely unexplored. Here, we generated a complete map of cell volumetric growth and deformation across the shoot apex in Arabidopsis thaliana . Within the organ-meristem boundary, we found that a subpopulation of cells next to fast-growing cells experiences volumetric shrinkage. To understand this process, we used a vertex-based model integrating mechanics and hydraulics, informed by the measured growth rates. Organ outgrowth simulations revealed the emerging water fluxes and predicted water deficit with volume loss for a few cells at the boundary. Consistently, in planta, a water-soluble dye is preferentially allocated to fast-growing tissues and fails to enter the boundary domain. Analysis of intact meristems further validated our model by revealing cell shrinkage next to fast-growing cells in different contexts of tissue surface curvature and cell deformation. A molecular signature of water deficit at the boundary further confirmed our conclusion. Taken together, we propose a model where the differential sink strength of emerging organs prescribes the hydraulic patterns that define the boundary domain at the shoot apex.
Fichier principal
Vignette du fichier
2023.08.27.554993v2.full.pdf (12.93 Mo) Télécharger le fichier
Origin Files produced by the author(s)

Dates and versions

hal-04254674 , version 1 (23-10-2023)

Identifiers

Cite

Juan Alonso-Serra, Ibrahim Cheddadi, Annamaria Kiss, Guillaume Cerutti, Claire Lionnet, et al.. Water fluxes contribute to growth patterning in shoot meristems. 2023. ⟨hal-04254674⟩
26 View
10 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More