Objective: The main goal of the athletes who undergo anterior cruciate ligament reconstruction (ACLR) surgery is a successful return-to-sport. At this stage, identifying muscular deficits becomes important. Hence, in this study, three discriminative features based on surface electromyographic signals (sEMG) acquired in a dynamic protocol are introduced to assess the damping ability and interpret activation patterns in lower-limb muscles of ACLR athletes. Methods: The features include the median frequency of the power spectrum density (PSD), the relative percentage of the equivalent damping or equivalent stiffness derived from the median frequency, and the energy of the signals in the time-frequency plane of the pseudo-Wigner-Ville distribution (PWVD). To evaluate the features, 11 healthy and 11 ACLR athletes (6 months post-reconstruction surgery) were recruited to acquire the sEMG signals from the medial and the lateral parts of the hamstrings, quadriceps, and gastrocnemius muscles in pre- and post-fatigue single-leg landings. Results: A significant damping deficiency is observed in the hamstring muscles of ACLR athletes by evaluating the proposed features. This deficiency indicates that more attention should be paid to this muscle of ACLR athletes in pre-return-to-sport rehabilitations. Conclusion: The quality of electromyography-based pre-return-to-sport assessments on ACLR subjects depends on the sEMG acquisition protocol, as well as the type and nature of the extracted features. Hence, combinatorial application of both energy-based features (derived from the PWVD) and power-based features (derived from the PSD) could facilitate the assessment process by providing additional biomechanical information regarding the behavior of the muscles surrounding the knee.