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Article Dans Une Revue Scientific Reports Année : 2018

Lectin-mediated protocell crosslinking to mimic cell-cell junctions and adhesion

Sarah Villringer
  • Fonction : Auteur
Josef Madl
  • Fonction : Auteur
Taras Sych
  • Fonction : Auteur
Christina Manner
  • Fonction : Auteur
Winfried Römer
  • Fonction : Auteur

Résumé

Cell adhesion is a crucial feature of all multicellular organisms, as it allows cells to organise themselves into tissues to carry out specific functions. Here, we present a mimetic approach that uses multivalent lectins with opposing binding sites to crosslink glycan-functionalised giant unilamellar vesicles. The crosslinking process drives the progression from contact puncta into elongated protocellular junctions, which form the vesicles into polygonal clusters resembling tissues. Due to their carbohydrate specificity, different lectins can be engaged in parallel with both natural and synthetic glycoconjugates to generate complex interfaces with distinct lectin domains. In addition, the formation of protocellular junctions can be combined with adhesion to a functionalised support by other ligand-receptor interactions to render increased stability against fluid flow. Furthermore, we consider that adhesion is a complex process of attraction and repulsion by doping the vesicles with a PEG-modified lipid, and demonstrate a dosedependent decrease of lectin binding and formation of protocellular junctions. We suggest that the engineering of prototissues through lectin-glycan interactions is an important step towards synthetic minimal tissues and in designing artificial systems to reconstruct the fundamental functions of biology.

Domaines

Chimie
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Dates et versions

hal-02106164 , version 1 (18-01-2021)

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Sarah Villringer, Josef Madl, Taras Sych, Christina Manner, Anne Imberty, et al.. Lectin-mediated protocell crosslinking to mimic cell-cell junctions and adhesion. Scientific Reports, 2018, 8 (1), ⟨10.1038/s41598-018-20230-6⟩. ⟨hal-02106164⟩
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