Two-photon calcium imaging from head-fixed Drosophila during optomotor walking behavior. - Inria - Institut national de recherche en sciences et technologies du numérique Access content directly
Journal Articles Nature Methods Year : 2010

Two-photon calcium imaging from head-fixed Drosophila during optomotor walking behavior.

Abstract

Drosophila melanogaster is a model organism rich in genetic tools to manipulate and identify neural circuits involved in specific behaviors. Here we present a technique for two-photon calcium imaging in the central brain of head-fixed Drosophila walking on an air-supported ball. The ball's motion is tracked at high resolution and can be treated as a proxy for the fly's own movements. We used the genetically encoded calcium sensor, GCaMP3.0, to record from important elements of the motion-processing pathway, the horizontal-system lobula plate tangential cells (LPTCs) in the fly optic lobe. We presented motion stimuli to the tethered fly and found that calcium transients in horizontal-system neurons correlated with robust optomotor behavior during walking. Our technique allows both behavior and physiology in identified neurons to be monitored in a genetic model organism with an extensive repertoire of walking behaviors.

Domains

Neuroscience

Dates and versions

hal-01062437 , version 1 (09-09-2014)

Identifiers

Cite

Johannes D Seelig, M Eugenia Chiappe, Gus K Lott, Anirban Dutta, Jason E Osborne, et al.. Two-photon calcium imaging from head-fixed Drosophila during optomotor walking behavior.. Nature Methods, 2010, 7 (7), pp.535-40. ⟨10.1038/nmeth.1468⟩. ⟨hal-01062437⟩
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