We present the PEASS Toolkit designed for the perceptual evaluation of audio source separation. It includes a MUSHRA-based listening-test protocol adapted for the subjective evaluation of audio source separation; a set of subjective measures - sounds and subjective scores - obtained by applying the proposed test protocol; and a set of auditory-motivated objective measures based on a new algorithm to decompose the estimation error into some distortion components (target distortion, interference, artefacts) and on integrating the outputs of the auditory-based objective measure PEMO-Q. This presentation will be the opportunity to detail the recent release of the PEASS toolkit, as well as its use in the Signal Separation Evaluation Campaign (SiSEC) in 2010.