With the increase of the complexity of automotive control, it is necessary to develop AEIICS (Automotive Electronic and Information Integrated Control System) for exchanging information between ECUs (Electronic Control Units) based on in-vehicle network technology. We named the parameter (or variable) contained in and transmitted by frame as signal. Period and deadline are basic temporal characteristics for signal and frame. Designers mainly focus on directly designing the period and deadline of frame but omit the temporal characteristic relationship between signal and frame. In this paper, we researched on the calculation method of period and deadline of frame according to the temporal characteristic of signal. Based on the period of signal, we proved the period of frame is the minimal period of signals composing this frame. Considering the possible time offset between the production time of data of signal and the first transmission time of frame containing this data, we deduced the calculation formula of deadline of frame by applying GCRT (Generalized Chinese Remainder Theorem).