In this paper, we investigate the effect of spatial and temporal resolution of cardiac MRI cine images on the extracted left ventricle motion. A previously developed and validated finite-element-based image registration method was used for the motion extraction. The study is performed on three subjects, for which the standard 2D cine stack (SA) in short axis orientation and 3D cine MRI series were acquired. The set of acquired image series was augmented by artificially constructed SA-like cine series created from the 3D cine images. Image series with several combinations of spatial and temporal resolution were evaluated for each subject. The study showed a strong correlation between the slice thickness and the accuracy of extracted displacement in the longitudinal direction. The effect of a lower temporal resolution was shown to be less significant. This could prove useful to update current guidelines for cardiac MRI acquisitions.