This research paper presents the design process of creating a user persona of Indian mothers of preterm neonates. Many of these preterm neonates require hospitalization in Neonatal Intensive Care Units (NICUs), leading to mental stress for mothers and their families. The main objective of mother’s persona is to understand the mother behavior and preferences for designing app for these mothers. The mother’s persona is proposed based on hypothesis, user interviews and data analysis. The result shows that the participant mothers of preterm neonates are graduates and homemakers from semi-urban areas around Pune, India. These mothers prefer non-vegetarian diet, they visit a pediatrician more frequently and presently. Moreover, the mothers have challenges with care and anxieties related to their neonates. They do not use any mobile healthcare app or YouTube videos for information about neonatal care. In the future, a mobile app will be developed for these mothers with due consideration to their user persona.