Studying the spatial distribution characters of rubber yield and soil nutrients and the rule of spatial variability are important for suitable fertilization strategy in rubber plantation. This paper selected Hongquan Branch, Guangba Branch and Gongai Branch of Guangba Farm in Hainan province as study area and total of 327 samples were selected in the rubber plantation. The spatial distribution characters of rubber yield and five soil nutrients, including organic matter (OM), total nitrogen (TN), available phosphorus (AP), available potassium (AK), exchangeable calcium (Ga), were studied using traditional analysis method and geo-statistics analysis method. The results showed that: (1) The average value of rubber yield was 3.55 kg/hm2 with moderate spatial variability and the average values of OM, N, P, K and Ga were 11.65 g/kg, 0.07%, 16.23 mg/kg, 49.65 mg/kg and 84.44 mg/kg, respectively. Soil OM, TN, AK and Ga had moderate spatial variability but AP had strong spatial variability. (2) Rubber yield and soil total nitrogen (N) nutrient had strong spatial dependence; soil OM, AP, AK and Ga had moderate spatial dependence. (3) Based on the previous reports of normal range of soil nutrients, soil OM and TN nutrient content were very low in the studied rubber plantation of Guangba Farm. Therefore, more nitrogen fertilizer should be applied in the rubber plantation in future.