Biomass is an important indicator to evaluate vegetation life activities and hyperspectral imagery from unmanned aerial vehicle (UAV) supplied with abundant texture features shows a great potential to estimate crop biomass. In this paper, principal component analysis (PCA) was used to select the principal component bands from UAV hyperspectral image. Eight texture features from the principal component bands were extracted by Gray Level Co-occurrence Matrix method, and the sensitive texture features were finally selected to construct the biomass estimation model. The results show that: (1) Texture features mean, ent, sm, hom, con, dis of the first principal component (pca1) and the mean of the third principal component (pca3) were significantly correlated with the biomass. (2) The biomass model by multiple texture features (R2 = 0.654, RMSE = 0.808 (103 kg/hm2)) demonstrated better fitting effect than that by single texture feature (R2 = 0.534, RMSE = 0.960 (103 kg/hm2)). The biomass estimation model based on the texture features of multiple principal components had a good fitting effect. Therefore, texture features of the UAV platform can accurately predict the winter wheat biomass.