It is investigated that nearly 86% containers were transferred by roads connection transport at Shenzhen port with the high-speed developing steps of Chinese economy. For a long time, Shenzhen container-trailer enterprises have provided essential and efficient logistics service for every dock. However, problems as operated by many bodies, small scale, decentralized business and poor competitiveness, etc. was universally existed. Because the port, big international logistics enterprises, policies, environment, competitors, customers, insurance companies and maintenance companies have great affection on the cost and income of container-trailer enterprises, this paper conducts a comprehensive analysis on operating environment of these enterprises by using the dynamic game theory of incomplete information to study some useful running modes of high efficiency and low cost operation, and also some feasible measures have been proposed.