The beef texture as marble is one of the important quality indexes of beef. Since the grading of beef marbling is largely determined by the subjective experience of the graders, there are inconsistencies and errors in judgment. Therefore, how to find objective and quantitative measure of the marbling abundance degree according to the grade standard of beef marbling has been one new study in the world meat science fields. We can use image analysis tools of Matlab to preprocess the image of beef marbling. Through data analysis, the percentage of image content marbled has been obtained, the detection model can be established through the construction of neural network, so it can lay the foundation for the prediction grade of the unknown kinds of beef marbling in the future.