The effect of agronomic factors including seedling age, transplanting density and net nitrogen ratio on the yield and profit of a single-cropping hybrid late rice (Oryza sativa) cultivar ‘Yongyou 12’ was investigated. The orthogonally rotational combination experimental design was used in setting field parameter test. Regression models assuming yield or profit as the objective function were constructed, and the impact from each individual or combinations of the three agronomic factors on the yield and profit of the crop was estimated. A computer simulation was performed to select the optimized agronomic scheme of growing rice ‘Yongyou 12’ under the field conditions used in this study.