This paper describes the measuring of pilot response time to a sudden change in a controlled parameter whilst flying an aircraft. The authors of this paper created an analytical model of human behavior from the basic data of an automated regulation. The measurements have been done on a Cessna 152 simulator at the University of Hertfordshire, Hatfield. The tested pilots were pilot students with several tens of flight hours in real planes. The pilot’s response to a sudden aircraft altitude change was measured. For analysis of the measured results a mathematical identification model in MATLAB® environment was used. The results obtained from MATLAB® confirm that the experimental measurements were successful.