The aim of this paper consists in presenting a new approach in studying the evolution of the business models of the categories of business actors belonging to a business Ecosystem focused on co-modal logistics networks. Each type of actors is pursuing its own value system on the basis of the key elements that identify and characterize the nature of its activities and business. The focus of the business models is on the key elements that characterize the value-creation links between the involved actors in the Value Chain. The paper focuses on broadening and exploring the traditional concepts of business models showing their evolution when considering the future business environment. To perform this analysis, a template, recalling the well-known approach by Osterwalder, is proposed, discussed and applied to systematically consider alternative approaches to value creation in terms of reference business models.