The purpose of this study is to evaluate the wearing comfort of uniform- shirts and to investigate the suitable value for the preset temperature of air conditioners thorough measuring material properties and psychophysiological responses. The uniform shirt was made of cellulose materials such as rayon and tencel for wearing in hot conditions like summers in Japan. Material properties measurement were made according to Kawabata Evaluation System (KES), Japan Industrial Standards (JIS) and Moisture Management Tester (MMT). Physiological response measurements were electrocardiogram (ECG), respiration, skin temperature and the humidity inside the clothes. Psychological response measurements were by semantic differential method and ranking method. We concluded that the uniform shirts made of rayon are comfortable even in hot conditions like summer in Japan. 29°C as the temperature setting of air conditioners is too hot for people. Respiration seems to be an important factor for evaluation of wearing comfort.