Wireless sensor networks (WSN) are increasingly popular in the field of greenhouse environment monitoring. In this paper, a new design of greenhouse remote monitoring system based on WSN and WEB is presented. The system has a three-tier structure of node layer, distributed management layer and WEB service layer. Experiments showed that the node layer has excellent self-organization and communication ability. The maximum distance of one-hop is 856 m. The distributed management layer can acquire and manage the data of temperature, humidity, light and the level of the carbon dioxide, and simultaneously send the data to WEB server database through the Internet. The WEB service layer can complete format storage, statistical analysis and conditional query for database, also ordinary users can obtain the graphic display of the greenhouse environment data through web browser. Normal working time of system reaches 141 days in the experiment. Meanwhile, normal operation rate exceeds 92%. Packet transfer rate of each sensor node is higher than 89.6% and lower than 95.4%.The greenhouse remote monitoring system based on WSN and WEB can realize an organic combination between distributed management and remote data sharing throughout different regions.