National e-Infrastructures are playing an increasingly important role in the support of complex computational and data requirements from all scientific disciplines, environmental informatics not excepting. Since 1996, such an e-Infrastructure is developed and operated in the Czech Republic, with its emphasis shifting from a shared uniform distributed infrastructure to a more user-tailored environment. Its development relates (and in some cases precedes) the evolution of European Grid Infrastructure (EGI), with its current vision of an Open Science Commons concept. While the current e-Infrastructure concept and its implementation is of a very generic nature, it can be tailored to specifically cover different needs of environmental applications. This paper gives an overview of the Czech national e-Infrastructure, its connection to EGI and a number of applications from environmental science domains.