Enterprise distributed real-time and embedded (DRE) publish/subscribe (pub/sub) systems manage resources and data that are vital to users. Cloud computing--where computing resources are provisioned elastically and leased as a service--is an increasingly popular deployment paradigm. Enterprise DRE pub/sub systems can leverage cloud computing provisioning services to execute needed functionality when on-site computing resources are not available. Although cloud computing provides flexible on-demand computing and networking resources, enterprise DRE pub/sub systems often cannot accurately characterize their behavior a priori for the variety of resource configurations cloud computing supplies (e.g., CPU and network bandwidth), which makes it hard for DRE systems to leverage conventional cloud computing platforms. This paper provides two contributions to the study of how autonomic configuration of DRE pub/sub middleware can provision and use on-demand cloud resources effectively. We first describe how supervised machine learning can configure DRE pub/sub middleware services and transport protocols autonomically to support end-to-end quality-of-service (QoS) requirements based on cloud computing resources. We then present results that empirically validate how computing and networking resources affect enterprise DRE pub/sub system QoS. These results show how supervised machine learning can configure DRE pub/sub middleware adaptively in < 10 μsec with bounded time complexity to support key QoS reliability and latency requirements.