EXOSC10/Rrp6 is essential for the eight-cell embryo/morula transition - Inria - Institut national de recherche en sciences et technologies du numérique Access content directly
Journal Articles Developmental Biology Year : 2022

EXOSC10/Rrp6 is essential for the eight-cell embryo/morula transition


The conserved 30-50 exoribonuclease EXOSC10/Rrp6 is required for gametogenesis, brain development, erythropoiesis and blood cell enhancer function. The human ortholog is essential for mitosis in cultured cancer cells. Little is known, however, about the role of Exosc10 during embryo development and organogenesis. We generated an Exosc10 knockout model and find that Exosc10(-/-) mice show an embryonic lethal phenotype. We demonstrate that Exosc10 maternal wild type mRNA is present in mutant oocytes and that the gene is expressed during all stages of early embryogenesis. Furthermore, we observe that EXOSC10 early on localizes to the periphery of nucleolus precursor bodies in blastomeres, which is in keeping with the protein's role in rRNA processing and may indicate a function in the establishment of chromatin domains during initial stages of embryogenesis. Finally, we infer from genotyping data for embryonic days e7.5, e6.5 and e4.5 and embryos cultured in vitro that Exosc10-/- mutants arrest at the eight-cell embryo/morula transition. Our results demonstrate a novel essential role for Exosc10 during early embryogenesis, and they are consistent with earlier work showing that impaired ribosome biogenesis causes a developmental arrest at the morula stage.
Fichier principal
Vignette du fichier
Petit et al-2021-EXOSC10-Rrp6 is essential for the eight-cell embryo-morula transition.pdf (5.37 Mo) Télécharger le fichier
Origin Files produced by the author(s)

Dates and versions

hal-03713237 , version 1 (13-07-2022)



Fabrice G. Petit, Soazik P. Jamin, Pierre-Yves Kernanec, Emmanuelle Becker, Guillaume Halet, et al.. EXOSC10/Rrp6 is essential for the eight-cell embryo/morula transition. Developmental Biology, 2022, 483, pp.58-65. ⟨10.1016/j.ydbio.2021.12.010⟩. ⟨hal-03713237⟩
115 View
108 Download



Gmail Mastodon Facebook X LinkedIn More