Comprehensive human urine standards for comparability and standardization in clinical proteome analysis
Résumé
Purpose-Urine proteomics is emerging as a powerful tool for biomarker discovery. The purpose of this study is the development of a well characterized "real life" sample that can be used as reference standard in urine clinical proteomics studies. Experimental design-We report on the generation of male and female urine samples that are extensively characterized by different platforms and methods (CE-MS, LC-MS, LC-MS/MS, GeLC-MS, and 2DE-MS) for their proteome and peptidome. In several cases analysis involved a definition of the actual biochemical entities, i.e. proteins/peptides associated with molecular mass and detected posttranslational modifications and the relative abundance of these compounds. Results-The combination of different technologies allowed coverage of a wide mass range revealing the advantages and complementarities of the different technologies. Application of these samples in "inter-laboratory" and "inter-platform" data comparison is also demonstrated.
Domaines
Sciences du Vivant [q-bio]
Fichier principal
2010, Mischak, Proteomics Clinical Applications.pdf (1.79 Mo)
Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)