Fragment-based modeling of protein-bound ssRNA - Inria - Institut national de recherche en sciences et technologies du numérique Accéder directement au contenu
Poster Année : 2016

Fragment-based modeling of protein-bound ssRNA

Modélisation par fragments des ARN simple-brin liés a une protéine

Résumé

Protein-RNA recognition supports many cellular functions, and abnormal protein-RNA interactions are crucial therapeutic targets in e.g. neurodegenerative diseases and RNA viruses infections. Moreover, synthetic RNA aptamers are used as protein modulators [ref in 1]. The rational conception of either aptamers or RNA-protein interaction inhibitors requires atomistic description of protein-RNA complexes. Yet their experimental resolution is arduous, and protein-RNA computational docking is hampered by the high flexibility of RNA single-stranded regions, which mostly provides recognition specificity. The lack of methodology for modeling ssRNA limits all protein-RNA docking methods [2]. We developed an original fragment-based approach, accurately predicting ssRNA-protein complexes structure from only protein structure and RNA sequence: We (i) cut the RNA sequence in overlapping trinucleotides, represented by sequence-specific conformers-ensembles in a fragment library built from known protein-RNA structures; (ii) dock each conformer-ensemble on the protein; (iii) select the spatially compatible poses; (iv) assemble them in a realistic conformation. Moreover, we developed and validated an RNA-protein contacts predictor, based on statistical analysis of known complexes, which provides (optional) starting points for the fragments docking. We applied them on ten complexes with various ssRNA sequences (6-11 nucleotides) and RNA-recognition domains. Without predicting specific contacts, we identify the RNA binding site more accurately than all existing methods [3]. Predicting 3-4 contacts, we model ssRNA within 1-2Å RMSD. Such crystallographic-like precision, never reached so far [1], reveals a methodological breakthrough in RNA-protein docking. [1] Chauvot-de-Beauchene et al (2016) NAR 44(10):4565-4580 [2] Fulle, Gohlke (2010) JMR 23(2):220-231 [3] Chauvot-de-Beauchene et al (2016) PloS.Comput.Biol. 12(1):e1004697
Fichier principal
Vignette du fichier
ECCB2017-A4.pdf (7.94 Mo) Télécharger le fichier
Abstract-ECCB2017.pdf (44.37 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-01573352 , version 1 (09-08-2017)

Identifiants

  • HAL Id : hal-01573352 , version 1

Citer

Isaure Chauvot de Beauchêne, Sjoerd J de Vries, Martin Zacharias. Fragment-based modeling of protein-bound ssRNA. ECCB 2016: The 15th European Conference on Computational Biology, Sep 2016, Den Haag, Netherlands. 2016. ⟨hal-01573352⟩
288 Consultations
94 Téléchargements

Partager

Gmail Facebook X LinkedIn More