Mastering Dendrimer Self-Assembly for Efficient siRNA Delivery: From Conceptual Design to In Vivo Efficient Gene Silencing - Inria - Institut national de recherche en sciences et technologies du numérique Accéder directement au contenu
Article Dans Une Revue Small Année : 2016

Mastering Dendrimer Self-Assembly for Efficient siRNA Delivery: From Conceptual Design to In Vivo Efficient Gene Silencing

Chao Chen
Paola Posocco
  • Fonction : Auteur
  • PersonId : 878654
Xiaoxuan Liu
  • Fonction : Auteur
Qiang Cheng
  • Fonction : Auteur
Erik Laurini
  • Fonction : Auteur
Jiehua Zhou
  • Fonction : Auteur
Cheng Liu
Yang Wang
  • Fonction : Auteur
  • PersonId : 986381
Jingjie Tang
  • Fonction : Auteur
Valentina Dal Col
  • Fonction : Auteur
Tianzhu Yu
  • Fonction : Auteur
Maurizio Fermeglia
  • Fonction : Auteur
  • PersonId : 878655
Fanqi Qu
  • Fonction : Auteur
Zicai Liang
  • Fonction : Auteur
John J. Rossi
  • Fonction : Auteur
Minghua Liu
  • Fonction : Auteur
Sabrina Pricl
  • Fonction : Auteur
  • PersonId : 878656

Résumé

Self-assembly is a fundamental concept and a powerful approach in molecular science. However, creating functional materials with the desired properties through self-assembly remains challenging. In this work, through a combination of experimental and computational approaches, the self-assembly of small amphiphilic dendrons into nanosized supramolecular dendrimer micelles with a degree of structural definition similar to traditional covalent high-generation dendrimers is reported. It is demonstrated that, with the optimal balance of hydrophobicity and hydrophilicity, one of the self-assembled nanomicellar systems, totally devoid of toxic side effects, is able to deliver small interfering RNA and achieve effective gene silencing both in cells - including the highly refractory human hematopoietic CD34(+) stem cells - and in vivo, thus paving the way for future biomedical implementation. This work presents a case study of the concept of generating functional supramolecular dendrimers via self-assembly. The ability of carefully designed and gauged building blocks to assemble into supramolecular structures opens new perspectives on the design of self-assembling nanosystems for complex and functional applications.

Domaines

Chimie

Dates et versions

hal-01459075 , version 1 (07-02-2017)

Identifiants

Citer

Chao Chen, Paola Posocco, Xiaoxuan Liu, Qiang Cheng, Erik Laurini, et al.. Mastering Dendrimer Self-Assembly for Efficient siRNA Delivery: From Conceptual Design to In Vivo Efficient Gene Silencing. Small, 2016, 12 (27), pp.3667-3676. ⟨10.1002/smll.201503866⟩. ⟨hal-01459075⟩
98 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More